Ca2+-sensing Transgenic Mice

Ca -sensing Transgenic Mice

Genetically encoded signaling proteins provide remarkable opportunities to design and target the expression of molecules that can be used to report critical cellular events in vivo, thereby markedly extending the scope and physiological relevance of studies of cell function. Here we report the development of a transgenic mouse expressing such a reporter and its use to examine postsynaptic signa...

Calcium Signaling in Transgenic Mice Overexpressing Cardiac Na+-Ca2+ Exchanger

We have produced transgenic mice which overexpress cardiac Na(+)-Ca2+ exchange activity. Overexpression has been assessed by Western blot, Northern blot, and immunofluorescence. Functional overexpression was analyzed using membrane vesicles and isolated ventricular myocytes. In whole cell clamped myocytes dialyzed with 0.1-0.2 mM Fura-2, the magnitude of ICa and Ca2+i-transient triggered by ICa...

Transgenic mice.

Introduction Transgenic is a word which is used to indicate an organism which has its genome altered. It is a synonym of genetically modified or recombinant organisms. These are generally created for human benefits. Transgenic animals are just one in a series of developments in the area of biotechnology. Biotechnology has transformed the way in which we understand processes such as engineering ...

transgenic mice

Normally, sonic hedgehog (Shh) signaling induces high levels of Patched 1 (Ptc1) and its associated transcription factor Gli1 with genesis of specific neuronal progeny. But their roles in the neural stem cells (NSCs), including glial precursor cells (GPCs), of Alzheimer’s disease (AD) are unclear. Here, we show that Ptc1 and Gli1 are significantly deficits in the hippocampus of an aged AD trans...

Development of Transgenic Mice Harboring Ovine Beta Lactoglobulin-Calcitonin Transgene